Laboratory buffer component worksheet calculator

Calculate stock transfer volumes and diluent volume with your own figures.

Your numbers

Use the units shown. Results update as you change valid inputs.

Use stock transfer volumes and diluent volume to compare changes to your inputs. The method and limits below explain the scope.

Examples

Examples

Try the starting example

Approved stocks [reagent,target concentration,stock concentration]
[["A",10,100],["B",5,50]]
Final buffer volume (mL)
100
Stock transfer volumes (mL)
[["A",10],["B",10]]
Diluent volume (mL)
80

Try another scenario

Approved stocks [reagent,target concentration,stock concentration]
[["A",10,100],["B",5,50]]
Final buffer volume (mL)
200
Stock transfer volumes (mL)
[["A",20],["B",20]]
Diluent volume (mL)
160
Calculation method

Calculation method

Stock transfer volumes (mL)

Each target×final volume/stock concentration; all concentrations must share consistent units per reagent.

Diluent volume (mL)

Final volume minus sum stock transfers; negative diluent rejected.

Symbols and units
a
Approved stocks [reagent,target concentration,stock concentration]
b
Final buffer volume (mL)

Use the units shown. A list keeps the order in which you enter its values. The symbol ^ means “raised to the power of”.

Assumptions & limits

Assumptions & limits

  • Volume arithmetic for a supplied approved recipe. Assumes additive liquid volumes and compatible concentration units; does not choose buffer chemistry or a protocol. Quantities are deterministic calculations from supplied observations and assumptions; no external data is fetched. Supported limits: Finite inputs only; numeric fields bounded as labelled. Lists and JSON records are limited to 256 entries unless a stricter tool bound is stated. No automatic design approval, legal conclusion, treatment recommendation or safety certification. Final buffer volume (mL): minimum 1e-12, maximum 1000000000000.0.
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