Laboratory nucleic acid amount calculator

Calculate total nucleic acid mass and full aliquots with your own figures.

Your numbers

Use the units shown. Results update as you change valid inputs.

Use total nucleic acid mass and full aliquots to compare changes to your inputs. The method and limits below explain the scope.

Examples

Examples

Try the starting example

Mass concentration (ng/µL)
50
Available volume (µL)
100
Aliquot volume (µL)
10
Total nucleic acid mass (ng)
5000
Full aliquots
10
Mass per full aliquot (ng)
500
Residual volume (µL)
0

Try another scenario

Mass concentration (ng/µL)
55.00000000000001
Available volume (µL)
100
Aliquot volume (µL)
10
Total nucleic acid mass (ng)
5,500
Full aliquots
10
Mass per full aliquot (ng)
550
Residual volume (µL)
0
Calculation method

Calculation method

Total nucleic acid mass (ng)

Concentration×volume.

Full aliquots

floor(volume/aliquot volume).

Mass per full aliquot (ng)

Concentration×aliquot volume.

Residual volume (µL)

Volume minus full aliquots×aliquot volume.

Symbols and units
a
Mass concentration (ng/µL)
b
Available volume (µL)
c
Aliquot volume (µL)

Use the units shown. A list keeps the order in which you enter its values. The symbol ^ means “raised to the power of”.

Assumptions & limits

Assumptions & limits

  • Measured concentration, volume. Returns Total mass and aliquot quantities. All coefficients and observations are user supplied; results describe only the stated mathematical model. Quantities are deterministic calculations from supplied observations and assumptions; no external data is fetched. Supported limits: Finite inputs only; numeric fields bounded as labelled. Lists and JSON records are limited to 256 entries unless a stricter tool bound is stated. No automatic design approval, legal conclusion, treatment recommendation or safety certification. Mass concentration (ng/µL): minimum 0, maximum 1000000000000.0. Available volume (µL): minimum 0, maximum 1000000000000.0. Aliquot volume (µL): minimum 1e-12, maximum 1000000000000.0.
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