Laboratory nucleic acid purity ratios calculator

Calculate blank-adjusted A260/A280 and blank-adjusted A260/A230 with your own figures.

Your numbers

Use the units shown. Results update as you change valid inputs.

Use blank-adjusted A260/A280 and blank-adjusted A260/A230 to compare changes to your inputs. The method and limits below explain the scope.

Examples

Examples

Try the starting example

A260 reading
1
A280 reading
0.55
A230 reading
0.5
Common blank absorbance
0.02
Blank-adjusted A260/A280
1.849057
Blank-adjusted A260/A230
2.041667
Adjusted absorbances
[0.98,0.53,0.48]

Try another scenario

A260 reading
1.1
A280 reading
0.55
A230 reading
0.5
Common blank absorbance
0.02
Blank-adjusted A260/A280
2.037736
Blank-adjusted A260/A230
2.25
Adjusted absorbances
[1.08,0.53,0.48]
Calculation method

Calculation method

Blank-adjusted A260/A280

(A260−blank)/(A280−blank).

Blank-adjusted A260/A230

(A260−blank)/(A230−blank).

Adjusted absorbances

Raw adjusted readings; ratios alone do not prove sample purity.

Symbols and units
a
A260 reading
b
A280 reading
c
A230 reading
d
Common blank absorbance

Use the units shown. A list keeps the order in which you enter its values. The symbol ^ means “raised to the power of”.

Assumptions & limits

Assumptions & limits

  • Absorbance readings, blank corrections. Returns Ratios and raw-data checks; no universal pass claim. All coefficients and observations are user supplied; results describe only the stated mathematical model. Quantities are deterministic calculations from supplied observations and assumptions; no external data is fetched. Supported limits: Finite inputs only; numeric fields bounded as labelled. Lists and JSON records are limited to 256 entries unless a stricter tool bound is stated. No automatic design approval, legal conclusion, treatment recommendation or safety certification. A260 reading: minimum None, maximum 1000000000000.0. A280 reading: minimum None, maximum 1000000000000.0. A230 reading: minimum None, maximum 1000000000000.0. Common blank absorbance: minimum None, maximum 1000000000000.0.
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