Laboratory plate normalization calculator

Calculate normalized response and control means and dynamic span with your own figures.

Your numbers

Use the units shown. Results update as you change valid inputs.

Use normalized response and control means and dynamic span to compare changes to your inputs. The method and limits below explain the scope.

Examples

Examples

Try the starting example

Well responses
0.1,0.3,0.6,0.9
Low-control replicate readings
0.1,0.12
High-control replicate readings
0.9,0.92
Normalized response (%)
[-1.25,23.75,61.25,98.75]
Control means and dynamic span
[0.11,0.91,0.8]

Try another scenario

Well responses
[0.2, 0.4, 0.7, 1]
Low-control replicate readings
[0.1, 0.12]
High-control replicate readings
[0.9, 0.92]
Normalized response (%)
[11.25,36.25,73.75,111.25]
Control means and dynamic span
[0.11,0.91,0.8]
Calculation method

Calculation method

Normalized response (%)

100(well−mean low)/(mean high−mean low); values outside0–100 preserved.

Control means and dynamic span

[low mean,high mean,span]; zero or negative span rejected.

Symbols and units
a
Well responses
b
Low-control replicate readings
c
High-control replicate readings

Use the units shown. A list keeps the order in which you enter its values. The symbol ^ means “raised to the power of”.

Assumptions & limits

Assumptions & limits

  • Well responses, control wells, supplied normalization rule. Returns Normalized responses and control checks. All coefficients and observations are user supplied; results describe only the stated mathematical model. Quantities are deterministic calculations from supplied observations and assumptions; no external data is fetched. Supported limits: Finite inputs only; numeric fields bounded as labelled. Lists and JSON records are limited to 256 entries unless a stricter tool bound is stated. No automatic design approval, legal conclusion, treatment recommendation or safety certification. Well responses: minimum None, maximum 1000000000000.0. Low-control replicate readings: minimum None, maximum 1000000000000.0. High-control replicate readings: minimum None, maximum 1000000000000.0.
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