Laboratory protein extinction concentration calculator

Calculate molar concentration and mass concentration with your own figures.

Your numbers

Use the units shown. Results update as you change valid inputs.

Use molar concentration and mass concentration to compare changes to your inputs. The method and limits below explain the scope.

Examples

Examples

Try the starting example

Blank-corrected absorbance
0.8
Optical path length (cm)
1
Supplied molar extinction coefficient (L/mol/cm)
50000
Protein molecular weight (g/mol)
50000
Molar concentration (mol/L)
0.000016
Mass concentration (mg/mL)
0.8

Try another scenario

Blank-corrected absorbance
0.8800000000000001
Optical path length (cm)
1
Supplied molar extinction coefficient (L/mol/cm)
50000
Protein molecular weight (g/mol)
50000
Molar concentration (mol/L)
0.000018
Mass concentration (mg/mL)
0.88
Calculation method

Calculation method

Molar concentration (mol/L)

Beer–Lambert c=A/(epsilon path).

Mass concentration (mg/mL)

Molar concentration×molecular weight; g/L equals mg/mL.

Symbols and units
a
Blank-corrected absorbance
b
Optical path length (cm)
c
Supplied molar extinction coefficient (L/mol/cm)
d
Protein molecular weight (g/mol)

Use the units shown. A list keeps the order in which you enter its values. The symbol ^ means “raised to the power of”.

Assumptions & limits

Assumptions & limits

  • Absorbance, path length, supplied coefficient. Returns Concentration under specified convention. All coefficients and observations are user supplied; results describe only the stated mathematical model. Quantities are deterministic calculations from supplied observations and assumptions; no external data is fetched. Supported limits: Finite inputs only; numeric fields bounded as labelled. Lists and JSON records are limited to 256 entries unless a stricter tool bound is stated. No automatic design approval, legal conclusion, treatment recommendation or safety certification. Blank-corrected absorbance: minimum 0, maximum 1000000000000.0. Optical path length (cm): minimum 1e-12, maximum 1000000000000.0. Supplied molar extinction coefficient (L/mol/cm): minimum 1e-12, maximum 1000000000000.0. Protein molecular weight (g/mol): minimum 1e-12, maximum 1000000000000.0.
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