Examples
Examples
Try the starting example
- Sample target Ct
- 24
- Sample reference Ct
- 20
- Calibrator target Ct
- 26
- Calibrator reference Ct
- 20
- Sample delta Ct
- 4
- Delta-delta Ct
- -2
- Relative expression fold change
- 4
Try another scenario
- Sample target Ct
- 26.400000000000002
- Sample reference Ct
- 20
- Calibrator target Ct
- 26
- Calibrator reference Ct
- 20
- Sample delta Ct
- 6.4
- Delta-delta Ct
- 0.4
- Relative expression fold change
- 0.757858
Calculation method
Calculation method
Sample delta Ct
Sample target−sample reference.
Delta-delta Ct
Sample deltaCt−calibrator deltaCt.
Relative expression fold change
2^(−deltaDeltaCt), assumes validated equal100% efficiencies.
Symbols and units
- a
- Sample target Ct
- b
- Sample reference Ct
- c
- Calibrator target Ct
- d
- Calibrator reference Ct
Use the units shown. A list keeps the order in which you enter its values. The symbol ^ means “raised to the power of”.
Assumptions & limits
Assumptions & limits
- Ct values, reference-target groups, efficiency assumption. Returns Relative expression under specified method. All coefficients and observations are user supplied; results describe only the stated mathematical model. Quantities are deterministic calculations from supplied observations and assumptions; no external data is fetched. Supported limits: Finite inputs only; numeric fields bounded as labelled. Lists and JSON records are limited to 256 entries unless a stricter tool bound is stated. No automatic design approval, legal conclusion, treatment recommendation or safety certification. Sample target Ct: minimum 0, maximum 60. Sample reference Ct: minimum 0, maximum 60. Calibrator target Ct: minimum 0, maximum 60. Calibrator reference Ct: minimum 0, maximum 60.
Are these inputs private?
These calculations run in your browser. A shared link contains the values you choose to share. Only questions submitted to the separate AI finder are sent to OpenAI.
Further reading
Background reading; this does not imply endorsement or independent review of SumFizz.