Laboratory serial transfer plate plan calculator

Calculate transfer map [well,transfer,diluent,concentration,source residual] and last-well remaining volume with your own figures.

Your numbers

Use the units shown. Results update as you change valid inputs.

Use transfer map [well,transfer,diluent,concentration,source residual] and last-well remaining volume to compare changes to your inputs. The method and limits below explain the scope.

Examples

Examples

Try the starting example

Starting stock concentration (user units)
100
Starting stock volume (µL)
1000
Serial wells [transfer from prior µL,total well volume µL]
[[100,1000],[100,1000],[100,1000]]
Transfer map [well,transfer,diluent,concentration,source residual]
[[1,100,900,10,900],[2,100,900,1,900],[3,100,900,0.1,900]]
Last-well remaining volume (µL)
1000

Try another scenario

Starting stock concentration (user units)
110.00000000000001
Starting stock volume (µL)
1000
Serial wells [transfer from prior µL,total well volume µL]
[[100,1000],[100,1000],[100,1000]]
Transfer map [well,transfer,diluent,concentration,source residual]
[[1,100,900,11,900],[2,100,900,1.1,900],[3,100,900,0.11,900]]
Last-well remaining volume (µL)
1000
Calculation method

Calculation method

Transfer map [well,transfer,diluent,concentration,source residual]

Each step withdraws transfer from previous well; add diluent to total, scale concentration by transfer/total.

Last-well remaining volume (µL)

Last well total volume, before any further withdrawal.

Symbols and units
a
Starting stock concentration (user units)
b
Starting stock volume (µL)
c
Serial wells [transfer from prior µL,total well volume µL]

Use the units shown. A list keeps the order in which you enter its values. The symbol ^ means “raised to the power of”.

Assumptions & limits

Assumptions & limits

  • Starting stock, specified dilution steps, well volumes. Returns Transfer-volume map and residual stock. All coefficients and observations are user supplied; results describe only the stated mathematical model. Quantities are deterministic calculations from supplied observations and assumptions; no external data is fetched. Supported limits: Finite inputs only; numeric fields bounded as labelled. Lists and JSON records are limited to 256 entries unless a stricter tool bound is stated. No automatic design approval, legal conclusion, treatment recommendation or safety certification. Starting stock concentration (user units): minimum 1e-12, maximum 1000000000000.0. Starting stock volume (µL): minimum 1e-12, maximum 1000000000000.0.
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