Examples
Examples
Try the starting example
- Template quantities or relative concentrations
- 1,10,100,1000
- Ct readings
- 30,26.68,23.36,20.04
- Ct versus log10 quantity slope
- -3.32
- Estimated PCR efficiency (%)
- 100.080525
- R squared
- 1
Try another scenario
- Template quantities or relative concentrations
- [1, 10, 100, 1000]
- Ct readings
- [31, 27.5, 24, 20.5]
- Ct versus log10 quantity slope
- -3.5
- Estimated PCR efficiency (%)
- 93.069773
- R squared
- 1
Calculation method
Calculation method
Ct versus log10 quantity slope
Linear regression Ct=m log10(quantity)+b.
Estimated PCR efficiency (%)
100(10^(−1/slope)−1); requires negative slope.
R squared
Fit diagnostic; no automatic assay acceptance.
Symbols and units
- a
- Template quantities or relative concentrations
- b
- Ct readings
Use the units shown. A list keeps the order in which you enter its values. The symbol ^ means “raised to the power of”.
Assumptions & limits
Assumptions & limits
- Template dilution factors, Ct readings. Returns Slope-derived efficiency estimate. All coefficients and observations are user supplied; results describe only the stated mathematical model. Quantities are deterministic calculations from supplied observations and assumptions; no external data is fetched. Supported limits: Finite inputs only; numeric fields bounded as labelled. Lists and JSON records are limited to 256 entries unless a stricter tool bound is stated. No automatic design approval, legal conclusion, treatment recommendation or safety certification. Template quantities or relative concentrations: minimum 1e-12, maximum 1000000000000.0. Ct readings: minimum None, maximum 1000000000000.0.
Are these inputs private?
These calculations run in your browser. A shared link contains the values you choose to share. Only questions submitted to the separate AI finder are sent to OpenAI.
Further reading
Background reading; this does not imply endorsement or independent review of SumFizz.